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活体转基因--卡钳电极

简要描述:

活体转基因--卡钳电极用于体内肌肉基因/药物传递电极。BTX Caliper 电极是一种体内可复用的卡钳状测径器电极,有多种应用,如在体内进行基因和药物的传递,也可经皮肤应用及在完整的植物上应用。

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活体转基因--卡钳电极384L 用于体内肌肉基因/药物传递电极

BTX Caliper电极是一种体内可复用的卡钳状测径器电极,有多种应用,如在体内进行基因和药物的传递,也可经皮肤应用及在完整的植物上应用。该电极由一个测径器和一对末端电极组成,共有两种类型:45-0101型的末端为一对1x1cm黄铜制末端电极,适用于小动物,45-0102型的末端为一对1.5x1.5cm或2x2cm的不锈钢制末端电极,适用于较大的区域表面,两电极间的距离可以调节。

活体转基因当把感兴趣的分子注射到体内以后,用电极板夹住相关目标组织,然后给出电脉冲,电场通过电极在组织细胞间形成暂时的孔道,使分子进入到与电极接触的组织细胞内。

The BTX Caliper Electrodes are in vivostyle re-usable electrodes that are used for a variety ofapplications. Caliper Electrodes come in two models, with eachmodel consisting of a caliper and a pair of end plates. Model 384are used for smaller animals with a smaller surface area, whilemodel 384L is used to target slightly larger surface areas. The endplates of the Caliper Electrode may be adjusted by using the blackroller mounted on the caliper. Caliper Electrodes may be cleanedwith a mild detergent and sterilized with ethanol or ethyleneoxide. Properly maintained Caliper Electrodes have an unlimitedlife, and are compatible with most BTX Electroporation Systems.

活体转基因Applications

Caliper Electrodes may be used for many electroporationapplications, including drug and gene delivery. The electrodes spanthe target area following injection of the molecule of interest,and electroporation pulses are delivered using a BTXElectroporation System, such as the ECM 600, ECM 630, ECM 830, ECM2001, or T820. The electric field introduced by the CaliperElectrodes cause transient pores to form in the cells of thetissue, allowing uptake of the molecules into the cells.Prud''Homme et al. used the Caliper electrodes to deliver plasmidDNA to skin for gene therapy studies on diabetes.1 Mir et al. usedthe Caliper Electrode for in vivo skeletal muscle work2 as well assubcutaneous tumor electrochemotherapy applications3. CaliperElectrodes can potentially be used for intact plant work aswell.4

Caliper Electrodes complement BTX Tweezertrodes Model 520, 522; BTXGenetrodes Model 508(S) and 510(S); and BTX 2 Needle ArrayElectrodes Model 530, 531, 532, 533 as part of the BTX in vivoelectrode line. Caliper Electrodes can be used to reproduceapplications requiring Tweezertrodes (transdermal) andGenetrodes/2-Needle.

Technical Specifications


Standard Capabilities
Voltage Range: 0-500V DC(depending on electrode gap)
Pulse Length Range: 1mses-10msec
Pulse Number Range: 1-99 (depending on voltage)
Physical Characteristics
Model 384 Electrode Dimensions: 1.0cm x 1.0cm
Model 384L Electrode Dimensions: 2.0cm x 2.0cm (1.5cm x 1.5cmspare pair included)
Electrode Material: Stainless Steel
Generator Compatibility: ECM 830, 600, 630, 2001, T820
Electrode gap: 0.1-13cm

Ordering Information

System Model Available Configurations
384 Caliper Electrodes, 1.0cm x 1.0cm Brass Plates
384L Caliper Electrodes, 2.0cm x 2.0cm Stainless Steel Plates(1.5cm x 1.5cm spare pair included)

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